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ATCC
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New England Biolabs
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New England Biolabs
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Sloning BioTechnology
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GenScript corporation
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Addgene inc
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Addgene inc
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GSL Biotech
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Addgene inc
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Image Search Results
Journal: PeerJ
Article Title: Development of a single-chain fragment variable fused-mutant HALT-1 recombinant immunotoxin against G12V mutated KRAS c olorectal cancer cells
doi: 10.7717/peerj.11063
Figure Lengend Snippet: Schematic structure of the recombinant immunotoxins in pET22b expression vector. m KRAS scFv and HALT-1 were connected via the glycine serine peptide linker. (A) scFv-mHALT-1: N-terminal scFv and C-terminal mHALT-1. (B) mHALT-1-scFv: N-terminal mHALT-1 and C-terminal scFv.
Article Snippet: The assembled recombinant immunotoxins were double digested with Xho I and Nco I (NEB, Hitchin, UK) and ligated into
Techniques: Recombinant, Expressing, Plasmid Preparation